Mast Cell-Derived SAMD14 Is a Novel Regulator of the Human Prostate Tumor Microenvironment

Mast cells (MCs) are important cellular components of the tumor microenvironment and significantly associated with poor patient outcomes in prostate cancer other solid cancers. The promotion progression partly involves heterotypic interactions between MCs cancer-associated fibroblasts (CAFs), which combine to potentiate a pro-tumor extracellular matrix promote epithelial cell invasion migration. Thus far, CAFs remain poorly understood. To identify molecular changes that may alter resident MC function microenvironment, we profiled transcriptome human isolated from patient-matched non-tumor tumor-associated regions fresh radical prostatectomy tissue. Transcriptomic profiling revealed distinct gene expression profile regions, including downregulation SAMD14, putative suppressor gene. Proteomic overexpression SAMD14 HMC-1 altered secretion proteins immune regulation processes. assess biological within model HMC-1-SAMD14+ conditioned media was added co-cultures primary prostatic epithelium. secretions were shown reduce deposition alignment produced by suppress pro-tumorigenic morphology. Overall, our data present first derived specimens identifies MC-derived as an mediator phenotype microenvironment.